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Biological imaging software tools

2012; Nature Portfolio; Volume: 9; Issue: 7 Linguagem: Inglês

10.1038/nmeth.2084

1548-7105

Kevin W. Eliceiri, Michael R. Berthold, I. Goldberg, Luis Ibáñez, B. S. Manjunath, Maryann E. Martone, Robert F. Murphy, Hanchuan Peng, Anne L. Plant, Badrinath Roysam, Nico Stuurman, Jason R. Swedlow, Pavel Tomančák, Anne E. Carpenter,

Advanced Fluorescence Microscopy Techniques

Representative members of the bioimage informatics community review the computational steps and some of the primary software tools available to biologists who are acquiring and analyzing microscopy-based digital image data, with a focus on open-source options. Few technologies are more widespread in modern biological laboratories than imaging. Recent advances in optical technologies and instrumentation are providing hitherto unimagined capabilities. Almost all these advances have required the development of software to enable the acquisition, management, analysis and visualization of the imaging data. We review each computational step that biologists encounter when dealing with digital images, the inherent challenges and the overall status of available software for bioimage informatics, focusing on open-source options.