The intracellular compartmentation of metabolites in isolated kidney cortex tubules
1980; Elsevier BV; Volume: 12; Issue: 1-2 Linguagem: Inglês
10.1016/0020-711x(80)90044-0
ISSN1878-6014
Autores Tópico(s)Metabolism and Genetic Disorders
Resumo1. A method is described to measure cytosolic and mitochondrial metabolites in isolated kidney tubule suspensions. 2. With the use of digitonin, 95% of cytosolic metabolites are released into the supernatant fraction whereas mitochondrial matrix enzymes are retained in the pellet fraction. 3. In a study of adaptation to acidosis, different α-oxoglutarate gradients between the tubular cell compartments are obtained, when medium pH is changed from 7.0 to 7.8.
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