Characterization of ultra-high molar mass hyaluronan: 1. Off-line static methods
1998; Elsevier BV; Volume: 39; Issue: 25 Linguagem: Inglês
10.1016/s0032-3861(98)00167-0
ISSN1873-2291
AutoresRaniero Mendichi, Alberto Giacometti Schieroni, Cesare Grassi, Alberto Re,
Tópico(s)Blood properties and coagulation
ResumoThis study concerns the problems encountered in the molecular characterization of ultra-high molar mass (UHMM) hyaluronan samples. The determination of the molar mass distribution of UHMM polymers is fairly difficult. Shear degradation and non-newtonian flow are the main difficulties in the fractionation on SEC columns. Considering these difficulties, the samples have remained previously characterized by off-line light scattering and viscometry. The characterization by means of light scattering presents the problem of non linear angular variation of the scattering. We present a method to estimate reliable values for molar mass and dimensions of UHMM hyaluronan polymers. To overcome the intrinsic viscosity shear rate dependence we have used a multi-bulb capillary viscometer. The persistence length of the UHMM hyaluronan polymer has been estimated.
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