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Glucose-limited fed-batch culture of Escherichia coli for production of recombinant human interleukin-2 with the DO-stat method

1992; Elsevier BV; Volume: 74; Issue: 3 Linguagem: Inglês

10.1016/0922-338x(92)90085-9

1872-8073

Seo, Dong Jin, Bong Hyun Chung, Young Do Hwang, Young Hoon Park,

thermodynamics and calorimetric analyses

Fed-batch culture employing the DO-stat method was carried out to produce recombinant human interleukin-2 in Escherichia coli. The concentration of interleukin-2 and final cell density in the fed-batch culture were 1.02 g/l and 60 OD (optical density), respectively. This represents a significant improvement in interleukin-2 production and final cell density over the equivalent values of batach fermentation.