Suppression of facilitative glucose transporter 1 mRNA can suppress tumor growth
2000; Elsevier BV; Volume: 154; Issue: 2 Linguagem: Inglês
10.1016/s0304-3835(00)00392-x
ISSN1872-7980
AutoresYoshikazu Noguchi, Aya Saito, Yohei Miyagi, Shoji Yamanaka, Doulet Marat, Chiharu Doi, Takaki Yoshikawa, Akira Tsuburaya, Takaaki Ito, Shinobu Satoh,
Tópico(s)Metabolism, Diabetes, and Cancer
ResumoWe attempted to suppress glucose transporter 1 (GLUT1) expression by transfecting MKN45 cells with cDNA for antisense GLUT1. Glucose transport was significantly decreased in cells with antisense GLUT1 compared with wild-type cells or cells with vector alone. Suppression of GLUT1 mRNA resulted in a decreased number of cells in the S phase. This was accompanied by overexpression of p21 protein. Tumorigenicity in the nude mice injected with antisense GLUT1 expressing cells was significantly slower than in those with wild-type MKN45 cells. These results suggest that antisense GLUT1 mRNA inhibits tumor growth through a G1 arrest and that expression of antisense GLUT1 mRNA via gene therapy can be used as a tool in the treatment of cancer.
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