Geographical characterization of hepatitis G virus genome: Evidence for HGV genotypes based on phylogenetic analysis
1998; Wiley; Volume: 10; Issue: 2 Linguagem: Inglês
10.1016/s1386-6346(97)00115-0
ISSN1872-034X
AutoresTamiko Saito, Teiichiro Shiino, Yasuyuki Arakawa, Shigeki Hayashi, Kenji Abe,
Tópico(s)Liver Disease Diagnosis and Treatment
ResumoAbstract Recently, the genome from a novel virus, termed hepatitis G virus (HGV) and GB virus C (GBV-C), has been discovered in the serum of patients. To investigate the heterogeneity of this viral genome, we sequenced 188 nucleotides at the 5′untranslated region (5′UTR) of HGV genome in 48 individuals from 11 different countries, including the USA, Asia (Japan, Korea, Nepal, Myanmar, Cambodia, Vietnam and Thailand), Spain, Egypt and Ghana and compared the results with these isolates. The data showed that there was a consensus genome sequence among the HGV isolates from each country and that it could be classified into at least three different groups or genotypes. Construction of phylogenetic trees, bootstrap analysis and calculation of mean distances between possible genotypes in the 5′UTR of HGV isolates also showed three major genotypes, which were defined as West Africa (type 1), US/Europe (type 2) and Asia types (type 3). Type 2 was found in various geographic areas, including USA, Europe, Egypt and Asia, while type 1 was found predominantly in West Africa. Type 3 was found mainly in Asia. Furthermore, type 3 could be clearly differentiated into a regional group, compared with the other two types, by bootstrap analysis. The HGV sequence of 5′UTR obtained from one Japanese patient showed no mutation over 12 years. These results suggest that HGV have genotypes corresponding to genetic heterogeneity and is correlated with geographic distribution.
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