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Addition of p -Azido- l -phenylalanine to the Genetic Code of Escherichia c oli

2002; American Chemical Society; Volume: 124; Issue: 31 Linguagem: Inglês

10.1021/ja027007w

1943-2984

Jason W. Chin, Stephen W. Santoro, Andrew B. Martin, David S. King, Lei Wang, Peter G. Schultz,

Click Chemistry and Applications

We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.