Photolabile linker for the solid-phase synthesis of base-sensitive oligonucleotides
1997; Elsevier BV; Volume: 38; Issue: 30 Linguagem: Inglês
10.1016/s0040-4039(97)01154-4
ISSN1873-3581
AutoresChristelle Dell’Aquila, Jean‐Louis Imbach, Bernard Rayner,
Tópico(s)DNA and Nucleic Acid Chemistry
Resumoo-Nitrophenyl-1,3-propanediol was covalently linked to long chain alkyl amine controlled pore glass (LCAA-CPG) beads through a stable phosphoramidate diester link. The resulting solid support was found suitable for the automated synthesis of 3′-phosphate unmodified as well as base-sensitive oligonucleotides. Efficient cleavage from the solid support was achieved by photolysis.
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