Gene therapy of the gut: introduction of the APC tumor-suppressor gene for cancer prevention or treatment
1995; Elsevier BV; Volume: 17; Issue: 3 Linguagem: Inglês
10.1016/0169-409x(95)00070-n
ISSN1872-8294
AutoresCarol A. Westbrook, Richard B. Arenas,
Tópico(s)Renal and related cancers
ResumoLiposomal delivery of gene therapy to the colonic epithelium has been evaluated in a rodent model. The use of β-galactosidase as a reporter gene demonstrated uptake and expression in virtually 100% of the epithelial cells which contacted the liposome-gene mixture. Expression was transient, persisting for 2–3 days, diminishing in accordance with normal epithelial turnover. Similar results were seen in both rats and mice. The method was used to deliver the APC tumor suppressor gene to rat colonic epithelium. Expression of RNA was verified by means of RNAse protection, as well as by an exon-connection strategy utilizing reverse-transcription. A 6-week period of bi-weekly retreatment established safety and minimal toxicity of this route; in particular, there were no obvious changes in cell growth or differentiation with prolonged administration of the APC gene. The potential use of this method for delivery of gene therapy, and the specific application of the APC gene for cancer prevention or treatment, are discussed.
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