Artigo Acesso aberto Produção Nacional Revisado por pares

Determinação de tanino em pedúnculo de caju: método da vanilina versus método do butanol ácido

2003; Brazilian Chemical Society; Volume: 26; Issue: 5 Linguagem: Inglês

10.1590/s0100-40422003000500022

ISSN

1678-7064

Autores

Tânia da Silveira Agostini-Costa, Andréa Lima, Marcelo Victor Lima,

Tópico(s)

Ginkgo biloba and Cashew Applications

Resumo

TANNIN IN CASHEW APPLE: VANILLIN VERSUS BUTANOL ACID ASSAY.To improve tannin assay in cashew apple, several parameters were examined, including (1) extraction solvents, (2) effects of water and boiling time on butanol acid reaction and (3) correlation between vanillin and butanol acid assay of tannin in cashew apples.The 50-70% acetone extracted the greatest amount of tannin from cashew apples.Concentrations of water in butanol reagents were adjusted and boiling time of butanol reaction was reduced at 15 min.Tannin of unripe cashew apples was purified on Sephadex LH-20, aiming to obtain tannin standard for butanol assay.The vanillin assay presented high correlation with the butanol acid assay.

Referência(s)