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Differentiation of Dried Sea Cucumber Products from Different Geographical Areas by Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry

2009; American Chemical Society; Volume: 57; Issue: 20 Linguagem: Inglês

10.1021/jf9018504

1520-5118

Zhongchen Wu, Huanwen Chen, Weiling Wang, Bin Jia, Tianlin Yang, Zhanfeng Zhao, Jianhua Ding, Xuxian Xiao,

Advanced Chemical Sensor Technologies

Without any sample pretreatment, mass spectral fingerprints of 486 dried sea cucumber slices were rapidly recorded in the mass range of m/z 50−800 by using surface desorption atmospheric pressure chemical ionization mass spectrometry (DAPCI-MS). A set of 162 individual sea cucumbers (Apostichopus japonicus Selenka) grown up in 3 different geographical regions (Weihai: 59 individuals, 177 slices; Yantai: 53 individuals, 159 slices; Dalian: 50 individuals, 150 slices;) in north China sea were successfully differentiated according to their habitats both by Principal Components Analysis (PCA) and Soft Independent Modeling of Class Analogy (SIMCA) of the mass spectral raw data, demonstrating that DAPCI-MS is a practically convenient tool for high-throughput differentiation of sea cucumber products. It has been found that the difference between the body wall tissue and the epidermal tissue is heavily dependent on the habitats. The experimental data also show that the roughness of the sample surface contributes to the variance of the signal levels in a certain extent, but such variance does not fail the differentiation of the dried sea cucumber samples.