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Supercritical-fluid extraction of aqueous samples and on-line coupling to supercritical-fluid chromatography

1989; Elsevier BV; Volume: 477; Issue: 1 Linguagem: Inglês

10.1016/s0021-9673(01)89629-1

1873-3778

Didier Thiébaut, Jean-Pierre Chervet, R.W. Vannoort, Gerhardus J. de Jong, U.A.Th. Brinkman, R.W. Frei,

Phase Equilibria and Thermodynamics

The potential of segmented-flow systems for on-line liquid/supercritical-fluid extraction was explored. The use of a phase separator (PS), its design and performance were investigated, utilizing phenol and 4-chlorophenol as the test compounds, water as the liquid phase and supercritical carbon dioxide as the extractant. On-line coupling of such a liquid/fluid extraction (SFE) system to supercritical fluid chromatography (SFC) was demonstrated, as was the feasibility of extracting phenol from an urine sample. The extraction efficiency for the test compounds was over 85%. The repeatability was about 8% relative standard deviation (R.S.D.) (n = 8) for the total SFE-PS-SFC system and 4% R.S.D. for both the SFE-PS and the SFC operation. The potential of coupling SFE to other chromatographic and detection principles is discussed.