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Effect of L-Arginine and L-NAME treatments on polymorphonuclear leukocytes and mononuclear cells influx during tumor growth

2009; Sociedade Brasileira Para o Desenvolvimento da Pesquisa em Cirurgia; Volume: 24; Issue: 2 Linguagem: Inglês

10.1590/s0102-86502009000200006

1678-2674

Olívia Teixeira Gomes Reis, Juliana Crepaldi Raini, Silvana Torossian Coradi, Dulce Helena Jardim Constantino,

Cancer, Hypoxia, and Metabolism

Evaluate polymorphonuclear leukocytes (PMN's) and mononuclear cells (MN's) involvement in the Ehrlich s solid tumor (ET) growth.90 Swiss mice were inoculated with 10(7) tumor cells (sc), distributed in three groups and treated once a day, via intraperitoneal (ip), with 0.1ml of diluent, L-Arginine (20mg/Kg) or L-NAME (20mg/Kg). After 7, 15 and 30 days of treatment, ten animals of each group were euthanized, the tumor mass was removed, processed and fixed for HE. Later, a morphometric analysis of the total area, parenchyma, necrosis, tumor stroma and PMN's leukocytes and MN's cells influx was performed.The L-Arginine treatment increased PMN's influx in the initial stage, whereas L-NAME reduced it. Our data suggests that NO effect on PMN's migration is dose-dependent. On the other hand, the MN s cells influx was reduced by L-NAME treatment at all evaluated periods and at the same periods an increase in tumor growth was observed.At initial stages of tumor implantation, both PMN's leukocytes and MN's cells act together to control ET development.