Portal de Periódicos da CAPES

Proton NMR of 15 N-Choline Metabolites Enhanced by Dynamic Nuclear Polarization

2009; American Chemical Society; Volume: 131; Issue: 44 Linguagem: Inglês

10.1021/ja9021304

1943-2984

Riddhiman Sarkar, Arnaud Comment, Paul R. Vasos, Sami Jannin, Rolf Gruetter, Geoffrey Bodenhausen, Hélène Hall, Deniz Kirik, Vladimir P. Denisov,

Electron Spin Resonance Studies

Chemical shifts of protons can report on metabolic transformations such as the conversion of choline to phosphocholine. To follow such processes in vivo, magnetization can be enhanced by dynamic nuclear polarization (DNP). We have hyperpolarized in this manner nitrogen-15 spins in 15N-labeled choline up to 3.3% by irradiating the 94 GHz electron spin resonance of admixed TEMPO nitroxide radicals in a magnetic field of 3.35 T during ca. 3 h at 1.2 K. The sample was subsequently transferred to a high-resolution magnet, and the enhanced polarization was converted from 15N to methyl- and methylene protons, using the small 2,3J(1H,15N) couplings in choline. The room-temperature lifetime of nitrogen polarization in choline, T1(15N) ≈ 200 s, could be considerably increased by partial deuteration of the molecule. This procedure enables studies of choline metabolites in vitro and in vivo using DNP-enhanced proton NMR.