The Analysis of Putrescine in Plant Samples by Automated Hplc
1981; Marcel Dekker; Volume: 4; Issue: 7 Linguagem: Inglês
10.1080/01483918108068811
ISSN2331-0413
AutoresDenis R. Lauren, Connie Parker, Michael Agnew, GH Smith,
Tópico(s)Mycotoxins in Agriculture and Food
ResumoAbstract The quantitative analysis of putrescine from plant tissue can be achieved using isocratic reversed-phase high performance liquid chromatography (HPLC). After preliminary extraction and clean up involving an ion exchange purification step, the isolated diamines are derivatised with benzoyl chloride for determination by HPLC. Automation of the HPLC step has led to a considerable saving in time for the total analysis. Potential problems associated with the analytical procedure are described.
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