Portal de Periódicos da CAPES

Identification of quantitative trait loci for canine hip dysplasia by two sequential multipoint linkage analyses

2012; Taylor & Francis; Volume: 39; Issue: 8 Linguagem: Inglês

10.1080/02664763.2012.673121

1360-0532

Lan Zhu, Su Chen, Zhuoxin Jiang, Zhiwu Zhang, Hung-Chih Ku, Xuesong Li, Melinda H. McCann, Stephen Harris, George Lust, Pual Jones, Rory J. Todhunter,

Veterinary Orthopedics and Neurology

Abstract Canine hip dysplasia (CHD) is characterized by hip laxity and subluxation that can lead to hip osteoarthritis. Studies have shown the involvement of multiple genetic regions in the expression of CHD. Although we have associated some variants in the region of fibrillin 2 with CHD in a subset of dogs, no major disease-associated gene has been identified. The focus of this study is to identify quantitative trait loci (QTL) associated with CHD. Two sequential multipoint linkage analyses based on a reversible jump Markov chain Monte Carlo approach were applied on a cross-breed pedigree of 366 dogs. Hip radiographic trait (Norberg Angle, NA) on both hips of each dog was tested for linkage to 21,455 single nucleotide polymorphisms across 39 chromosomes. Putative QTL for the NA was found on 11 chromosomes (1, 2, 3, 4, 7, 14, 19, 21, 32, 36, and 39). Identification of genes in the QTL region(s) can assist in identification of the aberrant genes and biochemical pathways involving hip dysplasia in both dogs and humans. Keywords: reversible jump Markov chain Monte Carlomultipoint linkage analysissingle nucleotide polymorphismquantitative trait locicanine hip dysplasia Acknowledgements The authors gratefully acknowledge the technical assistance of Dr Dana Brunson in running programs for data analysis in this study. The computational work was performed at the OSU High Performance Computing Center (OSUHPCC) at Oklahoma State University (OSU). We also thank the anonymous reviewers for their invaluable critical comments.