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Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy

2010; Optica Publishing Group; Volume: 18; Issue: 14 Linguagem: Inglês

10.1364/oe.18.014330

1094-4087

Liang Gao, Robert T. Kester, Nathan Hagen, Tomasz Tkaczyk,

Spectroscopy Techniques in Biomedical and Chemical Research

A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 x 285 x 60 (x, y, lambda). The spatial resolution is approximately 0.45 microm with a FOV of 100 x 100 microm(2). The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm. The channel's spectral resolution is approximately 8nm. The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.