Whole-Genome Random Sequencing and Assembly of Haemophilus influenzae Rd
1995; American Association for the Advancement of Science; Volume: 269; Issue: 5223 Linguagem: Inglês
10.1126/science.7542800
ISSN1095-9203
AutoresRobert Fleischmann, Mark D. Adams, Owen White, Rebecca A. Clayton, Ewen F. Kirkness, Anthony R. Kerlavage, Carol J. Bult, Jean-François Tomb, Brian Dougherty, Joseph M. Merrick, Keith McKenney, Granger Sutton, Will FitzHugh, Chris Fields, Jeannine D. Gocayne, John Scott, Robert B. Shirley, Li-lng Liu, Anna Glodek, Jenny M. Kelley, Janice F. Weidman, Cheryl A. Phillips, Tracy Spriggs, Eva Hedblom, Matthew Cotton, Teresa R. Utterback, Michael C. Hanna, David T. Nguyen, Deborah M. Saudek, Rhonda Brandon, Leah D. Fine, Janice L. Fritchman, Joyce L. Fuhrmann, N. S. M. Geoghagen, Cheryl L. Gnehm, Lisa McDonald, Keith V. Small, Claire M. Fraser, Hamilton O. Smith, J. Craig Venter,
Tópico(s)Biochemical and Structural Characterization
ResumoAn approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830,137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.
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