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Identification and Characterization of Novel Dermal Thy-1 Antigen-Bearing Dendritic Cells in Murine Skin

1996; Elsevier BV; Volume: 106; Issue: 3 Linguagem: Inglês

10.1111/1523-1747.ep12344049

1523-1747

Kunihiko Tamaki, Nami Yasaka, Chung‐Hsing Chang, Naoto OHTAKE, Atsushi Saitoh, Koichiro Nakamura, Masutaka Furue,

Dermatology and Skin Diseases

Using immunofluorescence on dermal sheets of mouse ear, we identified novel Thy-1+ dermal dendritic cells in murine skin. These cells are resistant to topical corticosteroid treatment. The number of these dermal Thy-1+ dendritic cells is not altered with aging of the mice or with 3 d of culture. Dermal Thy-1+ dendritic cells have melanosomes in their cytoplasm; hence, phagocytic activity is likely to be present. Double immunofluorescence revealed that most of these dermal Thy-1+ dendritic cells express CD45. Furthermore, conventional immunofluorescence and confocal laser scanning microscopic findings showed that most of these Thy-1+ dermal dendritic cells express gamma delta and V gamma 3 T-cell receptors. The relationship between these dermal Thy-1+ dendritic cells and dendritic epidermal T cells is discussed.