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In vitro photodynamic therapy on human oral keratinocytes using chloroaluminum-phthalocyanine

2008; Elsevier BV; Volume: 44; Issue: 11 Linguagem: Inglês

10.1016/j.oraloncology.2008.01.013

1879-0593

Erica Correio Coelho Tapajos, João Paulo Figueiró Longo, Andreza Ribeiro Simioni, Z.G.M. Lacava, Marcos Ferracioli Santos, P.C. Morais, Antônio Cláudio Tedesco, Ricardo Bentes Azevedo,

Nanoplatforms for cancer theranostics

In this study, oral carcinoma cells were used to evaluate chloroaluminum-phthalocyanine encapsulated in liposomes as the photosensitizer agent in support of photodynamic therapy (PDT). The genotoxicity and cytotoxicity behavior of the encapsulated photosensitizer in both dark and under irradiation using the 670-nm laser were investigated with the classical trypan blue cell viability test, the acridine orange/ethidium bromide staining organelles test, micronucleus formation frequency, DNA fragmentation, and cell morphology. The cell morphology investigation was carried out using light and electronic microscopes. Our findings after PDT include reduction in cell viability (95%) associated with morphologic alterations. The neoplastic cell destruction was predominantly started by a necrotic process, according to the assay with acridine orange and ethidium bromide, and this was confirmed by electronic microscopy analysis. Neither the PDT agent nor laser irradiation alone showed cytotoxicity, genotoxicity, or even morphologic alterations. Our results reinforce the efficiency of light-irradiated chloroaluminum-phthalocyanine in inducing a positive effect of PDT.