Production and secretion of recombinant soluble CD3 polypeptides by myeloma-derived transfectant clones
1989; Elsevier BV; Volume: 21; Issue: 3 Linguagem: Inglês
10.1016/0165-2478(89)90111-9
ISSN1879-0542
AutoresAlan Tunnacliffe, Carina Olsson, André Traunecker, G W Krissansen, K Karjalainen,
Tópico(s)Peptidase Inhibition and Analysis
ResumoSoluble forms of three human CD3 proteins have been produced by recombinant DNA techniques. The extracellular domain of CD3-gamma, -delta or -epsilon has been linked to the constant region of mouse immunoglobulin kappa light chain to form gamma-kappa, delta-kappa and epsilon-kappa chimaeric proteins. These are secreted by mouse myeloma-derived transfectant cell lines and are immunoprecipitable by CD3- or kappa-specific polyclonal antisera. Yields of 100-500 micrograms secreted recombinant proteins per litre of culture medium were obtained, which could be purified by anti-kappa affinity chromatography. The production of soluble CD3 illustrates the applicability of this technology to a loosely associated protein complex.
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