Identification of vitamin B12 and analogues by high- performance capillary electrophoresis and comparison with high-performance liquid chromatography
1992; Elsevier BV; Volume: 608; Issue: 1-2 Linguagem: Inglês
10.1016/0021-9673(92)87137-w
ISSN1873-3778
AutoresD Lambert, Charles Adjalla, F. Felden, S. Benhayoun, J. P. Nicolas, Jean‐Louis Guéant,
Tópico(s)Neonatal Health and Biochemistry
ResumoHigh-performance capillary electrophoresis (HPCE) was compared for the identification and determination of corrinoids (hydroxy-, cyano-, 5′-desoxyadenosyl- and methyl-cobalamin and cyano-cobinamide) with high-performance liquid chromatography (HPLC). The within-run reproducibility of the retention times in HPCE and HPLC were similar (2.4 and 2.2%, respectively). The detection limit in HPCE was 20 μg/ml. HPLC can be used, in combination with radioisotope dilution assay, when very low concentrations (100 pg/ml) have to be determined in biological material. HPCE is more efficient than HPLC for the identification of corrinoids after conversion into the CN-cobalamin and CN-cobinamide forms.
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