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Oligonucleotide‐arrayed TFT photosensor applicable for DNA chip technology

2006; Wiley; Volume: 95; Issue: 1 Linguagem: Inglês

10.1002/bit.20949

1097-0290

Tsuyoshi Tanaka, Keiichi Hatakeyama, Masahiro Sawaguchi, Akihito Iwadate, Yasushi Mizutani, Kazuhiro Sasaki, Naofumi Tateishi, Haruko Takeyama, Tadashi Matsunaga,

Advanced biosensing and bioanalysis techniques

Abstract A thin film transistor (TFT) photosensor fabricated by semiconductor integrated circuit (IC) technology was applied to DNA chip technology. The surface of the TFT photosensor was coated with TiO 2 using a vapor deposition technique for the fabrication of optical filters. The immobilization of thiolated oligonucleotide probes onto a TiO 2 ‐coated TFT photosensor using γ‐aminopropyltriethoxysilane (APTES) and N ‐(γ‐maleimidobutyloxy) sulfosuccinimide ester (GMBS) was optimized. The coverage value of immobilized oligonucleotides reached a plateau at 33.7 pmol/cm 2 , which was similar to a previous analysis using radioisotope‐labeled oligonucleotides. The lowest detection limits were 0.05 pmol/cm 2 for quantum dot and 2.1 pmol/cm 2 for Alexa Fluor 350. Furthermore, single nucleotide polymorphism (SNP) detection was examined using the oligonucleotide‐arrayed TFT photosensor. A SNP present in the aldehyde dehydrogenase 2 (ALDH2) gene was used as a target. The SNPs in ALDH2*1 and ALDH2*2 target DNA were detected successfully using the TFT photosensor. DNA hybridization in the presence of both ALDH2*1 and ALDH2*2 target DNA was observed using both ALDH2*1 and ALDH2*2 detection oligonucleotides‐arrayed TFT photosensor. Use of the TFT photosensor will allow the development of a disposable photodetecting device for DNA chip systems. © 2006 Wiley Periodicals, Inc.