Artigo Produção Nacional Revisado por pares

First report of Perkinsus beihaiensis in wild clams Anomalocardia brasiliana (Bivalvia: Veneridae) in Brazil

2014; Elsevier BV; Volume: 150; Linguagem: Inglês

10.1016/j.exppara.2014.07.012

ISSN

1090-2449

Autores

Liana Pinho Ferreira, Rachel Costa Sabry, Patrícia Mirella da Silva, Tarsis F. Gesteira, Lidiane de Souza Romão, Marcela Pinheiro Paz, Rubens Galdino Feijó, Maximiano Pinheiro Dantas Neto, Rodrigo Maggioni,

Tópico(s)

Marine Bivalve and Aquaculture Studies

Resumo

This is the first report of Perkinsus sp. (Bivalvia: Veneridae) infecting wild clams of the species Anomalocardia brasiliana in Brazil. The gill lamellae and rectum of 150 specimens of A. brasiliana collected in the Timonha river estuary (Ceará, Northeastern Brazil) in March 2012 were incubated in Ray's fluid thioglycollate medium (RFTM) for detection of Perkinsus sp. In RFTM, the prevalence of Perkinsus sp. was 14.7% (22/150) and the intensity of infection ranged from very light (1–10 cells across the slide) to light (12–100 cells). The presence of Perkinsus sp. was confirmed by PCR in seven (31.8%) out of 22 RFTM-positive specimens. DNA sequencing confirmed the presence of the genus Perkinsus and the phylogenetic analysis strongly indicated Perkinsus beihaiensis as the species responsible for the infection.

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