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TOR and PKA Signaling Pathways Converge on the Protein Kinase Rim15 to Control Entry into G0

2003; Elsevier BV; Volume: 12; Issue: 6 Linguagem: Inglês

10.1016/s1097-2765(03)00485-4

1097-4164

Ivo Pedruzzi, F Dubouloz, Elisabetta Cameroni, Valeria Wanke, Johnny Roosen, Joris Winderickx, Claudio De Virgilio,

Plant Gene Expression Analysis

The highly conserved Tor kinases (TOR) and the protein kinase A (PKA) pathway regulate cell proliferation in response to growth factors and/or nutrients. In Saccharomyces cerevisiae, loss of either TOR or PKA causes cells to arrest growth early in G1 and to enter G0 by mechanisms that are poorly understood. Here we demonstrate that the protein kinase Rim15 is required for entry into G0 following inactivation of TOR and/or PKA. Induction of Rim15-dependent G0 traits requires two discrete processes, i.e., nuclear accumulation of Rim15, which is negatively regulated both by a Sit4-independent TOR effector branch and the protein kinase B (PKB/Akt) homolog Sch9, and release from PKA-mediated inhibition of its protein kinase activity. Thus, Rim15 integrates signals from at least three nutrient-sensory kinases (TOR, PKA, and Sch9) to properly control entry into G0, a key developmental process in eukaryotic cells.