Molecular characterization of the mutable flaked allele for flower variegation in the common morning glory
1998; Wiley; Volume: 16; Issue: 3 Linguagem: Inglês
10.1046/j.1365-313x.1998.00308.x
ISSN1365-313X
AutoresYoshiki Habu, Yasuyo Hisatomi, Shigeru Iida,
Tópico(s)Plant Physiology and Cultivation Studies
ResumoSummary The mutable flaked (or a f ) lines of the common morning glory bear white flowers with colored flakes and sectors. The a f allele shows incomplete dominance. Plants in the heterozygous state A/a f bear lightly colored flowers with intensely colored flakes and occasionally with white sectors. We showed that the mutable a f allele is caused by insertion of a new transposable element, Tip100 , into the CHS‐D gene intron. Tip100 is 3.9 kb long and belongs to the Ac/Ds family. Although the timing and frequency of the flower variegation vary in different lines, they carry an identical mutable allele. We also noticed that a flaked subline, bearing variegated flowers, carries a Tip100 derivative, Tip100–1 . The structure of Tip100–1 contains an additional 48 bp terminal sequence as tandem repeats and its integration site is identical to that of Tip100 . Another line, with stable white flowers, is a double mutant carrying two copies of Tip100 in the CHS‐D gene. These results are discussed with regard to the variegated phenotypes of flowers in various mutable lines.
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