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PHYLOGENETIC POSITION OF THE STRIPED-LEGGED FORMS OF PANULIRUS LONGIPES (A. MILNE-EDWARDS, 1868) (DECAPODA, PALINURIDAE) INFERRED FROM MITOCHONDRIAL DNA SEQUENCES

2002; Brill; Volume: 75; Issue: 9 Linguagem: Inglês

10.1163/156854002763270455

1568-5403

Marie Antonette Juinio‐Meñez, Rachel Ravago,

Marine Biology and Ecology Research

[Morphological variation is observed among the striped-legged forms of Panulirus longipes (A. Milne-Edwards, 1868). Two forms have been described, a 'banded-whisker' and a 'white-whisker' form, which, until recently, were synonymized as Panulirus longipes femoristriga (Von Martens, 1872). In this study, analysis of partial nucleotide sequences of the mitochondrial cytochrome oxidase I gene provides molecular evidence supporting the recognition of the form Panulirus femoristriga (Von Martens, 1872) as a distinct species from the form Panulirus longipes bispinosus Borradaile, 1899. Nucleotide sequence divergence between the spotted-legged form P. longipes longipes (A. Milne-Edwards, 1868) and P. longipes bispinosus is estimated to be 3%. In contrast, sequence divergence between P. femoristriga and P.longipes bispinosus was estimated to be 17.8%, and is comparable to levels of genetic differentiation between other Panulirus species as well as among other crustacean taxa. The present result is concordant with previous analyses based on morphological characters and allozyme markers. A molecular phylogeny of selected Indo-Pacific Panulirus species is also presented, and is likewise broadly consistent with phylogenetic relationships inferred from morphological characters. The sympatric occurrence of P.longipes longipes, P.longipes bispinosus, and P.femoristriga in the Philippines is reported. La variation morphologique est observee parmi les formes a pattes rayees de Panulirus longipes (A. Milne-Edwards, 1868). Deux formes ont ete decrites, une forme banded-whisker et une forme white-whisker qui, jusqu'a present, etaient considerees comme synonymes de Panulirus longipes femoristriga (Von Martens, 1872). Au cours de cette etude, l'analyse des sequences partielles de nucleotides du gene mitochondrial du cytochrome oxydase I a fourni la preuve moleculaire appuyant la reconnaissance de la forme white-whisker Panulirus longipes femoristriga (Von Martens, 1872) comme distincte de la forme banded-whisker Panulirus longipes bispinosus Borradaile, 1899. La divergence des sequences nucleotidiques entre la forme a pattes tachetees P.longipes longipes (A. Milne-Edwards, 1868) et P.longipes bispinosus est estimee a 3%. En revanche, la divergence des sequences entre P.femoristriga et P.longipes bispinosus a ete estimee a 17,8%, et est comparable au niveau de differentiation genetique entre d'autres especes de Panulirus aussi bien que parmi d'autres taxons de crustaces. Le present resultat est concordant avec des analyses precedentes fondees sur les caracteres morphologiques et les marqueurs allozymes. Une phylogenie moleculaire d'une serie d'especes choisies de Panulirus indo-pacifiques est aussi presentee, et est aussi largement en accord avec les relations phylogenetiques etablies a partir des caracteres morphologiques. La presence sympatrique de P. longipes longipes, P. longipes bispinosus, et P.femoristriga aux Philippines est rapportee., Morphological variation is observed among the striped-legged forms of Panulirus longipes (A. Milne-Edwards, 1868). Two forms have been described, a 'banded-whisker' and a 'white-whisker' form, which, until recently, were synonymized as Panulirus longipes femoristriga (Von Martens, 1872). In this study, analysis of partial nucleotide sequences of the mitochondrial cytochrome oxidase I gene provides molecular evidence supporting the recognition of the form Panulirus femoristriga (Von Martens, 1872) as a distinct species from the form Panulirus longipes bispinosus Borradaile, 1899. Nucleotide sequence divergence between the spotted-legged form P. longipes longipes (A. Milne-Edwards, 1868) and P. longipes bispinosus is estimated to be 3%. In contrast, sequence divergence between P. femoristriga and P.longipes bispinosus was estimated to be 17.8%, and is comparable to levels of genetic differentiation between other Panulirus species as well as among other crustacean taxa. The present result is concordant with previous analyses based on morphological characters and allozyme markers. A molecular phylogeny of selected Indo-Pacific Panulirus species is also presented, and is likewise broadly consistent with phylogenetic relationships inferred from morphological characters. The sympatric occurrence of P.longipes longipes, P.longipes bispinosus, and P.femoristriga in the Philippines is reported. La variation morphologique est observee parmi les formes a pattes rayees de Panulirus longipes (A. Milne-Edwards, 1868). Deux formes ont ete decrites, une forme banded-whisker et une forme white-whisker qui, jusqu'a present, etaient considerees comme synonymes de Panulirus longipes femoristriga (Von Martens, 1872). Au cours de cette etude, l'analyse des sequences partielles de nucleotides du gene mitochondrial du cytochrome oxydase I a fourni la preuve moleculaire appuyant la reconnaissance de la forme white-whisker Panulirus longipes femoristriga (Von Martens, 1872) comme distincte de la forme banded-whisker Panulirus longipes bispinosus Borradaile, 1899. La divergence des sequences nucleotidiques entre la forme a pattes tachetees P.longipes longipes (A. Milne-Edwards, 1868) et P.longipes bispinosus est estimee a 3%. En revanche, la divergence des sequences entre P.femoristriga et P.longipes bispinosus a ete estimee a 17,8%, et est comparable au niveau de differentiation genetique entre d'autres especes de Panulirus aussi bien que parmi d'autres taxons de crustaces. Le present resultat est concordant avec des analyses precedentes fondees sur les caracteres morphologiques et les marqueurs allozymes. Une phylogenie moleculaire d'une serie d'especes choisies de Panulirus indo-pacifiques est aussi presentee, et est aussi largement en accord avec les relations phylogenetiques etablies a partir des caracteres morphologiques. La presence sympatrique de P. longipes longipes, P. longipes bispinosus, et P.femoristriga aux Philippines est rapportee.]