Conversion of Proliferation and Production of the Colony Stimulating Factor during Serial Passage of Mouse Fibroblasts in Culture
1979; Japan Society of Cell Biology; Volume: 4; Issue: 4 Linguagem: Inglês
10.1247/csf.4.267
ISSN1347-3700
AutoresT Matsumura, Shigeyo Y. Miyashita, Tadao Ohno,
Tópico(s)Virus-based gene therapy research
ResumoFibroblast-like cells from a BALB/c mouse embryo were propagated and transferred weekly in culture with a constant inoculation density of 5 × 103 cells/cm2. In this culture schedule a period of decreased proliferation (crisis) continued for 3 to 4 weeks before the initiation of continuous cell proliferation. This provided an experimental system suitable for studying proliferation conversion. The number and the proliferation rate of clonogenic cells increased early in crisis. Production of the colony-stimulating factor for mouse bone marrow cells by fibroblast-like cells was enhanced during the period of decreasing proliferation and crisis, then it decreased when continuous proliferation began. The results indicate that cells with infinite proliferation potential appear early in crisis, although the major population of cells terminates proliferation during crisis and enhances differentiated functions.
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