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Surface Association and Uptake of poly(lactic-co-glycolic) Acid Nanoparticles By Aspergillus Flavus

2014; Future Science Ltd; Volume: 5; Issue: 11 Linguagem: Inglês

10.4155/tde.14.85

2041-6008

Erica S Muse, Nipur R Patel, Carlos E. Astete, K. E. Damann, Cristina M. Sabliov,

Nanoparticle-Based Drug Delivery

To study the interaction of fluorescently tagged nanoparticles with Aspergillus flavus.Covalently tagged poly(lactic-co-glycolic) acid (PLGA) nanoparticles (PLGA-tetramethylrhodamine [PLGA-TRITC]), and PLGA-TRITC with entrapped coumarin-6 (double-tagged) nanoparticles, were synthesized using an oil-in-water emulsion evaporation method. Nanoparticle interaction with A. flavus was assessed using fluorescent microscopy.PLGA-TRITC nanoparticles associated with the surface of fungal spores and hyphae, with limited fluorescence observed within the interior. With double-tagged nanoparticles, comparatively more red fluorescence (TRITC) was measured on the fungal surface and more green (coumarin-6) on the interior, resulting from uptake of released coumarin-6.The majority of nanoparticles associated with the fungal surface, while smaller nanoparticles were internalized. Surface association between polymeric nanoparticles and A. flavus may facilitate content uptake.