Artigo Acesso aberto Revisado por pares

The IGF-1/PI3K/Akt Pathway Prevents Expression of Muscle Atrophy-Induced Ubiquitin Ligases by Inhibiting FOXO Transcription Factors

2004; Elsevier BV; Volume: 14; Issue: 3 Linguagem: Inglês

10.1016/s1097-2765(04)00211-4

ISSN

1097-4164

Autores

Trevor N. Stitt, Doreen Drujan, Brian Clarke, Frank J. Panaro, Yekatarina Timofeyva, William O. Kline, Michael Gonzalez, George D. Yancopoulos, David J. Glass,

Tópico(s)

Sirtuins and Resveratrol in Medicine

Resumo

Abstract Skeletal muscle size depends upon a dynamic balance between anabolic (or hypertrophic) and catabolic (or atrophic) processes. Previously, no link between the molecular mediators of atrophy and hypertrophy had been reported. We demonstrate a hierarchy between the signals which mediate hypertrophy and those which mediate atrophy: the IGF-1/PI3K/Akt pathway, which has been shown to induce hypertrophy, prevents induction of requisite atrophy mediators, namely the muscle-specific ubiquitin ligases MAFbx and MuRF1. Moreover, the mechanism for this inhibition involves Akt-mediated inhibition of the FoxO family of transcription factors; a mutant form of FOXO1, which prevents Akt phosphorylation, thereby prevents Akt-mediated inhibition of MuRF1 and MAFbx upregulation. Our study thus defines a previously uncharacterized function for Akt, which has important therapeutic relevance: Akt is not only capable of activating prosynthetic pathways, as previously demonstrated, but is simultaneously and dominantly able to suppress catabolic pathways, allowing it to prevent glucocorticoid and denervation-induced muscle atrophy.

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