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USE OF GREEN FLUORESCENT PROTEIN TO MEASURE TUMOR GROWTH IN AN IMPLANTED BLADDER TUMOR MODEL

2002; Lippincott Williams & Wilkins; Volume: 167; Issue: 2 Part 1 Linguagem: Inglês

10.1016/s0022-5347(01)69134-1

1527-3792

Suzy V. Torti, MEG GOLDEN-FLEET, Mark C. Willingham, Rong Ma, Mark Cline, YOSHIRO SAKIMOTO, Frank M. Torti,

Cancer, Hypoxia, and Metabolism

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Feb 2002USE OF GREEN FLUORESCENT PROTEIN TO MEASURE TUMOR GROWTH IN AN IMPLANTED BLADDER TUMOR MODEL SUZY V. TORTI, MEG GOLDEN-FLEET, MARK C. WILLINGHAM, RONG MA, MARK CLINE, YOSHIRO SAKIMOTO, and FRANK M. TORTI SUZY V. TORTISUZY V. TORTI More articles by this author , MEG GOLDEN-FLEETMEG GOLDEN-FLEET More articles by this author , MARK C. WILLINGHAMMARK C. WILLINGHAM More articles by this author , RONG MARONG MA More articles by this author , MARK CLINEMARK CLINE More articles by this author , YOSHIRO SAKIMOTOYOSHIRO SAKIMOTO More articles by this author , and FRANK M. TORTIFRANK M. TORTI More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)69134-1AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Bladder cancer is a common malignancy in which local recurrence and distant failure contribute to poor patient outcome. The search for improved therapies remains a high priority in this disease. For most experimental therapies animal tumor models represent an important step between in vitro testing and clinical trials. A useful animal model of bladder cancer involves the orthotopic implantation of bladder tumor cells in sygeneic animals. This model offers the opportunity to test the efficacy of therapies administered systemically or intravesically. However, quantitation of tumor growth has been difficult. Materials and Methods: To allow the quantitative assessment of tumor mass in this model and differentiate tumor cells from normal bladder epithelium at early stages of tumor growth we transfected cells of the MBT2 murine bladder cancer cell line with a vector encoding enhanced green fluorescent protein and devised an enzyme-linked immunosorbent assay that enables the detection of green fluorescent protein in cells at the pg. level. Results: Using this assay tumor growth can be detected 7 days after implantation and by 14 days levels of green fluorescent protein are more than 500-fold greater than in controls. Conclusions: Combined with the enzyme-linked immunosorbent assay use of this MBT2 green fluorescent protein transfectant allows tumor cell growth to be monitored with sensitivity and reproducibility. It reduces the time required to measure effects on tumor growth to 2 weeks, while preserving the advantages of this orthotopic tumor model. References 1 : Cancer statistics, 2000. 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Crossref, Medline, Google Scholar From the Departments of Biochemistry, Cancer Biology, Pathology and Medicine and Comprehensive Cancer Center, Wake Forest University School of Medicine, Winston-Salem, North Carolina© 2002 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited by Al‐Gubory K (2019) Shedding light on fibered confocal fluorescence microscopy: Applications in biomedical imaging and therapiesJournal of Biophotonics, 10.1002/jbio.201900146, VOL. 12, NO. 11, Online publication date: 1-Nov-2019. Gills J, Moret R, Zhang X, Nelson J, Maresh G, Hellmers L, Canter D, Hudson M, Halat S, Matrana M, Marino M, Reiser J, Shuh M, Laborde E, Latsis M, Talwar S, Bardot S and Li L (2018) A patient-derived orthotopic xenograft model enabling human high-grade urothelial cell carcinoma of the bladder tumor implantation, growth, angiogenesis, and metastasisOncotarget, 10.18632/oncotarget.26024, VOL. 9, NO. 66, (32718-32729), Online publication date: 24-Aug-2018. Chade D, Andrade P, Borra R, Leite K, Andrade E, Villanova F and Srougi M (2008) Histopathological characterization of a syngeneic orthotopic murine bladder cancer modelInternational braz j urol, 10.1590/S1677-55382008000200013, VOL. 34, NO. 2, (220-229), Online publication date: 1-Mar-2008. LUO Y, CHEN X and O'DONNELL M (2018) USE OF PROSTATE SPECIFIC ANTIGEN TO MEASURE BLADDER TUMOR GROWTH IN A MOUSE ORTHOTOPIC MODELJournal of Urology, VOL. 172, NO. 6 Part 1, (2414-2420), Online publication date: 1-Dec-2004.TANAKA M, GEE J, CERDA J, ROSSER C, ZHOU J, BENEDICT W and GROSSMAN H (2018) Noninvasive Detection of Bladder Cancer in an Orthotopic Murine Model With Green Fluorescence Protein CytologyJournal of Urology, VOL. 170, NO. 3, (975-978), Online publication date: 1-Sep-2003. Yoshida D, Watanabe K, Noha M, Takahashi H, Teramoto A and Sugisaki Y (2003) Anti-invasive Effect of an Anti-Matrix Metalloproteinase Agent in a Murine Brain Slice Model Using the Serial Monitoring of Green Fluorescent Protein-labeled Glioma CellsNeurosurgery, 10.1227/00006123-200301000-00024, VOL. 52, NO. 1, (187-197), Online publication date: 1-Jan-2003. Yoshida D, Watanabe K, Noha M, Takahashi H, Teramoto A and Sugisaki Y (2003) Anti-invasive Effect of an Anti-Matrix Metalloproteinase Agent in a Murine Brain Slice Model Using the Serial Monitoring of Green Fluorescent Protein-labeled Glioma CellsNeurosurgery, 10.1097/00006123-200301000-00024, VOL. 52, NO. 1, (187-197), Online publication date: 1-Jan-2003. Yoshida D, Watanabe K, Noha M, Takahashi H, Teramoto A and Sugisaki Y (2002) Tracking cell invasion of human glioma cells and suppression by anti-matrix metalloproteinase agent in rodent brain-slice modelBrain Tumor Pathology, 10.1007/BF02478930, VOL. 19, NO. 2, (69-76), Online publication date: 1-Sep-2002. Volume 167Issue 2 Part 1February 2002Page: 724-728 Advertisement Copyright & Permissions© 2002 by American Urological Association, Inc.Keywordsbladder neoplasmsfluorescent antibody techniquemicebladdertumor markers, biologicalMetricsAuthor Information SUZY V. TORTI More articles by this author MEG GOLDEN-FLEET More articles by this author MARK C. WILLINGHAM More articles by this author RONG MA More articles by this author MARK CLINE More articles by this author YOSHIRO SAKIMOTO More articles by this author FRANK M. TORTI More articles by this author Expand All Advertisement PDF downloadLoading ...