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Immunoreactivity of Synthetic Peptides Derived from Proteins of Cryptococcus Gattii

2014; Future Medicine; Volume: 9; Issue: 7 Linguagem: Inglês

10.2217/fmb.14.49

1746-0921

Rafael Melo Santos de Serpa Brandão, Liline Maria Soares Martins, Hélida Monteiro de Andrade, Angélica Rosa Faria, Maria José Soares Leal, Adalberto Socorro da Silva, Bodo Wanke, Márcia dos Santos Lazéra, Marilene Henning Vainstein, Rinaldo Pôncio Mendes, Daniela Vanessa Moris, Ricardo de Souza Cavalcante, Semíramis Jamil Hadad do Monte,

Antifungal resistance and susceptibility

Aim: To determine the immunoreactivity of synthetic Cryptococcus-derived peptides. Materials & methods: A total of 63 B-cell epitopes from previously identified Cryptococcus gattii immunoreactive proteins were synthesized and evaluated as antigens in ELISAs. The peptides were first evaluated for their ability to react against sera from immunocompetent subjects carrying cryptococcal meningitis. Peptides that yielded high sensitivity and specificity in the first test were then retested with sera from individuals with other fungal pathologies for cross-reactivity determination. Results: Six of 63 synthetic peptides were recognized by antibodies in immunoassays, with a specificity of 100%, sensitivity of 78% and low cross-reactivity. Conclusion: We successfully determined the immunoreactivity of selected synthetic peptides of C. gattii derived proteins.