New colored and fluorescent amine substrates for activated fibrin stabilizing factor (Factor XIIIa) and for transglutaminase
1983; Elsevier BV; Volume: 131; Issue: 2 Linguagem: Inglês
10.1016/0003-2697(83)90193-8
ISSN1096-0309
AutoresL. Lóránd, K. N. Parameswaran, Pauline T. Velasco, L.K.-H. Hsu, Gerald E. Siefring,
Tópico(s)Hemoglobin structure and function
ResumoTwo fluorescent (FITC and 6-chloro-2-methoxyacridine) and an intensely colored (dabsyl) derivative of cadaverine were synthesized, following earlier work from this laboratory with dansylcadaverine, in order to enlarge the scope of possibilities for labeling some γ-glutamine sites in proteins. Enzyme affinities of the amine substrates for human Factor XIIIa and for guinea pig liver transglutaminase were measured. The utility of dabsylcadaverine was further demonstrated by activity staining of these enzymes, following electrophoresis in agarose, and by measuring the Factor XIII zymogen of human plasma colorimetrically.
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