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Laser Myocardial Revascularization Modulates Expression of Angiogenic, Neuronal, and Inflammatory Cytokines in a Porcine Model of Chronic Myocardial Ischemia

2010; Wiley; Volume: 17; Issue: 5 Linguagem: Inglês

10.1111/j.1540-8191.2001.tb01171.x

1540-8191

Shmuel Fuchs, Richard Baffour, Yoram Vodovotz, Matie Shou, Eugenio Stabile, Fermin O. Tio, Martin B. Leon, Ran Kornowski,

Pain Management and Treatment

Journal of Cardiac SurgeryVolume 17, Issue 5 p. 413-424 Laser Myocardial Revascularization Modulates Expression of Angiogenic, Neuronal, and Inflammatory Cytokines in a Porcine Model of Chronic Myocardial Ischemia Shmuel Fuchs M.D., Corresponding Author Shmuel Fuchs M.D. Washington Hospital Center, Washington, DCCardiovascular Research Institute, Washington Hospital Center, 110 Irving Street N.W., Suite 4B-1, Washington D.C. 20010. Fax: 202–877–2715; e-mail: [email protected]Search for more papers by this authorRichard Baffour Ph.D, Richard Baffour Ph.D Washington Hospital Center, Washington, DCSearch for more papers by this authorYoram Vodovotz Ph.D, Yoram Vodovotz Ph.D Washington Hospital Center, Washington, DCSearch for more papers by this authorMatie Shou M.D., Matie Shou M.D. Washington Hospital Center, Washington, DCSearch for more papers by this authorEugenio Stabile M.D., Eugenio Stabile M.D. Washington Hospital Center, Washington, DCSearch for more papers by this authorFermin O. Tio M.D.,, Fermin O. Tio M.D., Biomedical Research Foundation of South Texas, Inc., San Antonio, TexasSearch for more papers by this authorMartin B. Leon M.D.,, Martin B. Leon M.D., Lenox Hill Hospital, New York, New YorkSearch for more papers by this authorRan Kornowski M.D., Ran Kornowski M.D. Washington Hospital Center, Washington, DCSearch for more papers by this author Shmuel Fuchs M.D., Corresponding Author Shmuel Fuchs M.D. Washington Hospital Center, Washington, DCCardiovascular Research Institute, Washington Hospital Center, 110 Irving Street N.W., Suite 4B-1, Washington D.C. 20010. Fax: 202–877–2715; e-mail: [email protected]Search for more papers by this authorRichard Baffour Ph.D, Richard Baffour Ph.D Washington Hospital Center, Washington, DCSearch for more papers by this authorYoram Vodovotz Ph.D, Yoram Vodovotz Ph.D Washington Hospital Center, Washington, DCSearch for more papers by this authorMatie Shou M.D., Matie Shou M.D. Washington Hospital Center, Washington, DCSearch for more papers by this authorEugenio Stabile M.D., Eugenio Stabile M.D. Washington Hospital Center, Washington, DCSearch for more papers by this authorFermin O. Tio M.D.,, Fermin O. Tio M.D., Biomedical Research Foundation of South Texas, Inc., San Antonio, TexasSearch for more papers by this authorMartin B. Leon M.D.,, Martin B. Leon M.D., Lenox Hill Hospital, New York, New YorkSearch for more papers by this authorRan Kornowski M.D., Ran Kornowski M.D. Washington Hospital Center, Washington, DCSearch for more papers by this author First published: 12 July 2010 https://doi.org/10.1111/j.1540-8191.2001.tb01171.xCitations: 10AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Abstract Background: Controversy exists whether transmyocardial laser revascularization (TMR) is associated with angiogenesis or neuromodulation and whether these are time-dependent phenomena. Accordingly, we performed a time-course analysis of the expression of angiogenic and neuronal factors following experimental percutaneous TMR. Methods and Results: Five weeks after placing ameroid constrictors on the circumflex coronary artery, 16 pigs underwent left ventricular mapping guided TMR using Ho:YAG laser (2 J × 1 pulse) at 30 sites directed at the ischemic zones and 11 animals were ischemic controls. Histology and immunostaining were obtained at 1 and 2 weeks (4 TMR and 3 controls at each time point) and at 4 weeks (8 TMR and 5 controls) for vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), nerve growth factor (βNGF), substance P (SP), and monocyte chemoattractant protein-1 (MCP-1). Immunoreactivity was scored using a digital image analysis system. Factor VIII staining was used for blood vessel counting. Enhanced regional expression of VEGF, bFGF and MCP-1 in the TMR group was noted at 1 and 2 weeks with a threefold increase at 4 weeks following TMR compared to controls. βNGF expression in the TMR group was enhanced at 1 and 2 weeks with subsequent decline at 4 weeks to the controls level. SP expression was not significantly different between groups at all time points. There was a twofold increase in the number of blood vessels in the TMR group at 4 weeks, which was not apparent earlier. Conclusions: These immunohistological findings suggest that cytokines expression compatible with angiogenesis and neuromodulation occurs early after TMR. Up-regulation of angiogenic and inflammatory cytokines may be more sustained than neuromodulation. 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