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Macrophage inhibitory factor-A3 derived from Mycobacterium avium serovar 2 inhibits candidacidal activity of murine peritoneal macrophages

1996; Elsevier BV; Volume: 53; Issue: 3-4 Linguagem: Inglês

10.1016/s0378-1135(96)01220-5

1873-2542

Murray E. Hines, Carolyn Cray, François Elvinger, Norman H. Altman,

Veterinary medicine and infectious diseases

Macrophage inhibitory factor-A3 (MIF-A3), a fraction derived from Mycobacterium avium serovar 2 inhibited candidacidal activity in macrophages from C57BL6, C57BL10, C3HHeJ and AJ strains of mice. Inhibition of candidacidal activity was demonstrated at MIF-A3 concentrations ranging from 100–400 μg/ml in macrophages without additional stimulators (exception C3HHeJ macrophages) and in macrophages additionally stimulated with 200 U/ml interferon-γ, 100 ng/ml phorbol myristate acetate and 0.4 ng/ml E. coli lipopolysaccharide from all mouse strains tested. The decreased candidacidal effect produced by MIF-A3 was dose-dependent and appeared greatest in macrophages treated with phorbol myristate acetate and lipopolysaccharide. This effect was neutralized by the addition of goat anti-MIF-A3 antiserum. Macrophages from the Bcgs mouse strains (C57BL6 and C57BL10) were more sensitive to the effect(s) of MIF-A3 than macrophages from the Bcgr mouse strains (C3HHeJ and AJ).