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IMPROVED CONTRACTILITY OF OBSTRUCTED BLADDERS AFTER TADENAN TREATMENT IS ASSOCIATED WITH REVERSAL OF ALTERED MYOSIN ISOFORM EXPRESSION

2000; Lippincott Williams & Wilkins; Volume: 163; Issue: 6 Linguagem: Inglês

10.1016/s0022-5347(05)67619-7

1527-3792

Cristiano Mendes Gomes, Michael E. DiSanto, Patrick Horan, Robert M. Levin, Alan J. Wein, Samuel Chacko,

Kidney Stones and Urolithiasis Treatments

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Jun 2000IMPROVED CONTRACTILITY OF OBSTRUCTED BLADDERS AFTER TADENAN TREATMENT IS ASSOCIATED WITH REVERSAL OF ALTERED MYOSIN ISOFORM EXPRESSION CRISTIANO M. GOMES, MICHAEL E. DISANTO, PATRICK HORAN, ROBERT M. LEVIN, ALAN J. WEIN, and SAMUEL CHACKO CRISTIANO M. GOMESCRISTIANO M. GOMES , MICHAEL E. DISANTOMICHAEL E. DISANTO , PATRICK HORANPATRICK HORAN , ROBERT M. LEVINROBERT M. LEVIN , ALAN J. WEINALAN J. WEIN , and SAMUEL CHACKOSAMUEL CHACKO View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)67619-7AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Tadenan is a plant extract from Pygeum africanum used in the treatment of benign prostatic hyperplasia, to protect the bladder from contractile dysfunction induced by partial bladder outlet obstruction (BOO). The aim of the present study was to determine whether the Tadenan-induced return of detrusor contractility affects the expression of myosin isoforms, which differ at the C-terminal (SM1 and SM2) and the N-terminal regions (SM-A and SM-B). Materials and Methods: Four groups of New Zealand White rabbits (3 to 5 kg., 4 to 6 rabbits per group) were either partially obstructed by ligation of the urethra (groups 1 and 2) or not obstructed (groups 3 and 4). After 2 weeks, rabbits from groups 2 and 4 received Tadenan in peanut oil (vehicle) orally at 100 mg./kg./day for 3 weeks and rabbits in groups 1 and 3 received vehicle only. Rabbits were sacrificed and bladders were removed and weighed. Contractility studies were performed on isolated strips of detrusor and the remaining muscular layer from the bladder body was used to study the expression of myosin heavy chain (MHC) isoforms at mRNA (SM1, SM2, SM-A, and SM-B) and the protein (SM1 and SM2) levels by RT-PCR and SDS-PAGE analyses, respectively. Results: Tadenan significantly reduced the effect of BOO on bladder mass. The diminished contractile response to field stimulation and carbachol secondary to urethral obstruction was significantly reversed by Tadenan treatment. The relative ratios for MHC isoforms were altered at the mRNA (SM2:SM1 and SM-A:SM-B) and protein (SM2:SM1) levels in obstruction. Upon treatment with Tadenan, the ratio of these isoforms returned to normal, as shown at the mRNA levels. In addition, the altered relative ratio of SM2:SM1 at the protein level also returned to nearly normal values after treatment. Conclusions: Improvement of obstruction-induced contractile dysfunction of the detrusor following treatment with Tadenan is associated with changes in the expression of myosin isoforms. The alteration in the expression of myosin isoforms associated with obstruction-induced hypertrophy is reversed close to normal in the detrusor smooth muscle from Tadenan-treated obstructed rabbits. References 1 : Efficacy of Pygeum africanum extract in the medical therapy of urination disorders due to benign prostatic hyperplasia: evaluation of objective and subjective parameters. A placebo-controlled double-blind multicenter study. Wiener Klinische Wochenschrift1990; 102: 667. Google Scholar 2 : A clinical and urodynamic study of Tadenan in the treatment of benign prostatic hypertrophy. Eur Urol1977; 3: 218. Google Scholar 3 : Controlled study of the effects of Pygeum africanum extract on the functional symptoms of prostatic adenoma. Annales d' Urologie1984; 18: 193. 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Google Scholar From the Department of Pathobiology and Division of Urology, University of Pennsylvania, Philadelphia, Pennsylvania, and the College of Pharmacy, Union University, Albany, New York© 2000 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byNOZAKI K, TOMIZAWA K, YOKOYAMA T, KUMON H and MATSUI H (2018) Calcineurin Mediates Bladder Smooth Muscle Hypertrophy After Bladder Outlet ObstructionJournal of Urology, VOL. 170, NO. 5, (2077-2081), Online publication date: 1-Nov-2003.LEVIN R, HASS M, BELLAMY F, HORAN P, WHITBECK K, CHOW P, KUNG L and GOSLING J (2018) Effect of Oral Tadenan Treatment on Rabbit Bladder Structure and Function After Partial Outlet ObstructionJournal of Urology, VOL. 167, NO. 5, (2253-2259), Online publication date: 1-May-2002.PAREKH M, LOBEL R, O'CONNOR L, LEGGETT R and LEVIN R (2018) PROTECTIVE EFFECT OF VITAMIN E ON THE RESPONSE OF THE RABBIT BLADDER TO PARTIAL OUTLET OBSTRUCTIONJournal of Urology, VOL. 166, NO. 1, (341-346), Online publication date: 1-Jul-2001. Volume 163Issue 6June 2000Page: 2008-2013 Advertisement Copyright & Permissions© 2000 by American Urological Association, Inc.Keywordsobstructionbladder contractilitymyosinTadenanMetrics Author Information CRISTIANO M. GOMES More articles by this author MICHAEL E. DISANTO More articles by this author PATRICK HORAN More articles by this author ROBERT M. LEVIN More articles by this author ALAN J. WEIN More articles by this author SAMUEL CHACKO More articles by this author Expand All Advertisement PDF downloadLoading ...