Dihydrofolate reductase induced by folic acid in cultured human cells
1973; Elsevier BV; Volume: 293; Issue: 1 Linguagem: Inglês
10.1016/0005-2744(73)90401-4
ISSN1878-1454
AutoresBrian L. Hillcoat, L. Marshall, John B. Patterson,
Tópico(s)Molecular Biology Techniques and Applications
ResumoCells of human origin in suspension culture increased their dihydrofolate reductase activity during logarithmic growth. High concentrations of folic acid caused a further increase in this activity. The effect of folic acid seemed specific since general protein synthesis and the activity of three other enzymes were not altered in its presence. The folate-induced increase required protein and RNA synthesis. The effects of inhibitors of RNA synthesis suggested that ribosomal RNA, rather than messenger RNA, might be limiting during the induction period.
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