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Determination of amphetamine, methamphetamine and dimethamphetamine in Human Urine by Solid-Phase Microextraction (SPME)-Gas Chromatography/Mass Spectrometry

1998; Elsevier BV; Volume: 716; Issue: 1-2 Linguagem: Inglês

10.1016/s0378-4347(98)00304-1

1872-812X

Seung-Woon Myung, Hye-Ki Min, Seung-Ki Kim, Myung-Soo Kim, Jin-Byom Cho, Taek-Jae Kim,

Analytical Chemistry and Chromatography

A simple and rapid assay method for three stimulant drugs (amphetamine, methamphetamine, and dimethamphetamine) in human urine using solid-phase microextraction was developed. In solid-phase microextraction, the drugs were equilibrated between the adsorbent coated-fiber and aqueous sample matrix. After adsorption of the analytes, the fiber was directly transferred to the injector of a gas chromatograph, where the analytes were thermally desorbed and subsequently separated by the gas chromatograph and detected by mass spectrometer. The solid-phase microextraction method, which did not require solvents, was found to be a fast and simple analytical method. We optimized the solid-phase microextraction technique, for factors such as the NaCl salt effect (30%), pH effect (pH=12.4), equilibration time (30 min), desorption time (1 min) and coated-fiber type (100 μm poly(dimethylsiloxane)) and detected the stimulants in human urine, obtained from human subjects. The detection limits of each drug were below 1–10 ng/ml. The developed method can be applied to the abused drug test.