TRANSMISSION OF A GENETICALLY ENGINEERED RHIZOBACTERIUM BY GRASSHOPPERS IN THE LABORATORY AND FIELD
1999; Wiley; Volume: 9; Issue: 1 Linguagem: Inglês
10.1890/1051-0761(1999)009[0245
ISSN1939-5582
AutoresWilliam E. Snyder, David W. Tonkyn, Daniel A. Kluepfel,
Tópico(s)Evolution and Genetic Dynamics
ResumoEcological ApplicationsVolume 9, Issue 1 p. 245-253 Article TRANSMISSION OF A GENETICALLY ENGINEERED RHIZOBACTERIUM BY GRASSHOPPERS IN THE LABORATORY AND FIELD William E. Snyder, William E. Snyder Department of Biological Sciences, Clemson University, Clemson, South Carolina 29634 USASearch for more papers by this authorDavid W. Tonkyn, David W. Tonkyn Department of Biological Sciences, Clemson University, Clemson, South Carolina 29634 USASearch for more papers by this authorDaniel A. Kluepfel, Daniel A. Kluepfel Department of Plant Pathology and Physiology, Clemson University, Clemson, South Carolina 29634 USASearch for more papers by this author William E. Snyder, William E. Snyder Department of Biological Sciences, Clemson University, Clemson, South Carolina 29634 USASearch for more papers by this authorDavid W. Tonkyn, David W. Tonkyn Department of Biological Sciences, Clemson University, Clemson, South Carolina 29634 USASearch for more papers by this authorDaniel A. Kluepfel, Daniel A. Kluepfel Department of Plant Pathology and Physiology, Clemson University, Clemson, South Carolina 29634 USASearch for more papers by this author First published: 01 February 1999 https://doi.org/10.1890/1051-0761(1999)009[0245:TOAGER]2.0.CO;2Citations: 8Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Root-colonizing bacteria have little inherent dispersal ability. However, we have shown previously that they routinely move into plant stems, sometimes at high densities, and might therefore be acquired and transmitted by foliage-feeding insects. Here we describe laboratory and field experiments on the transmission of a genetically engineered strain of the root-colonizing bacterium Pseudomonas chlororaphis (formerly aureofaciens) by a common herbivorous insect, the red-legged grasshopper Melanoplus femurrubrum. In laboratory and field microcosms, the engineered P. chlororaphis were applied to corn seeds at planting and allowed to colonize the developing roots and to invade the aerial tissues naturally. Adult M. femurrubrum that later fed on the foliage of these plants became infested with the bacteria. The bacteria were harbored throughout the digestive tracts and elsewhere in the exposed grasshoppers and could be recovered from their frass. Grasshoppers carried the bacteria for ≥1 wk after removal from the source of inoculum and could transmit the bacteria to new plants. In the laboratory, the likelihood of transmission declined as the proportion of grasshoppers remaining infested decreased through time. Transmission was less predictable in the field. Transport by insects may make naturally occurring soil bacteria more mobile than previously thought and could make the containment of engineered strains unlikely. However, at least in this system, microbial movement through plants and insects in the field was accurately predicted by laboratory studies, which therefore remain useful in basic research in microbial ecology, and in risk assessment. Literature Cited Angle, J. S., M. A. Levin, J. V. Gagliardi, and M. S. McIntosh . 1995. Validation of microcosms for examining the survival of Pseudomonas aureofaciens (lacZY) in soil. Applied and Environmental Microbiology 61: 2835–2839. 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