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BIBTEX RIS

Extraction, partial purification and characterization of a bacteriocin (fragilicin) produced by a strain of Bacteroides fragilis isolated from Callithrix penicillata

1994; Elsevier BV; Volume: 145; Issue: 1 Linguagem: Inglês

10.1016/0923-2508(94)90062-0

ISSN

1769-7123

Autores

Luiz M. Farias, Antônio Helvécio Tótola, Cláudia Madeira Miranda, Marcelo Addas‐Carvalho, Carlos Américo Veiga Damasceno, C. A. P. Tavares, E. O. Cisalpino, Eliane Vieira,

Tópico(s)

Microbial Natural Products and Biosynthesis

Resumo

A strain of Bacteroides fragilis, isolated from the marmoset Callithrix penicillata, produced protein(s) with bacteriocin activity (fragicilin). Two active fractions (36 and 150 kDa) were isolated by chromatography. The bacteriocin exhibited iso- and heteroantagonism. It remained stable between pH 3 and 10 and at 60 degrees C for 24 h. Pronase, trypsin, proteinase K and type VII protease inactivated the bacteriocin, giving evidence of its protein nature.

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