Induction of beta-2-microglobulin release in vitro by chronic lymphocytic leukaemia cells: relation to total protein synthesis
1999; Elsevier BV; Volume: 23; Issue: 10 Linguagem: Inglês
10.1016/s0145-2126(99)00109-5
ISSN1873-5835
AutoresJuhani Vilpo, Leena Vilpo, Mikko Hurme, Pauli Vuorinen,
Tópico(s)Immunodeficiency and Autoimmune Disorders
ResumoThe in vitro production of beta2-M by B-CLL cells from 27 patients was investigated. In all cases, low spontaneous beta2-M release was observed. The production of beta2-M was enhanced to various extents when induced with 13 different stimulants and their combinations including IL-2, TNFalpha, SAC and TPA. Beta2-M release was 3.8-fold (range from 1.9 to 9.2-fold) in cultures stimulated with TPA (10 ng/ml), compared with the spontaneous release, and even faster if TNFalpha or IL-2 were added. A strong correlation was revealed between beta2-M production and the total protein synthesis of leukaemic cells when the latter was assessed using 14C-L-leucine incorporation. Hence, both beta2-M release and leucine incorporation are promising activation markers for CLL B-lymphocytes.
Referência(s)