Produção Nacional
Revisado por pares
Microarray‐based gene expression analysis of human osteoblasts in response to different biomaterials
2008; Wiley; Volume: 88A; Issue: 2 Linguagem: Inglês
10.1002/jbm.a.31701
ISSN1552-4965
AutoresKarina Fittipaldi Bombonato‐Prado, L.S. Bellesini, Cristina M. Junta, Márcia Martins Marques, Geraldo Aleixo Silva Passos, Adalberto Luiz Rosa,
Tópico(s)Dental materials and restorations
ResumoAbstract Several biomaterials have been widely used in bone regeneration/substitution procedures in orthopedic and oral surgery. However, how these biomaterials alter osteoblast gene expression is poorly understood. We therefore attempted to address this question by using cDNA microarray technique to identify genes that are differentially regulated in osteoblasts exposed to biomaterials comprehending the biocompatibility spectrum of bioactive (bioglass and hydroxyapatite), bioinert (Ti and stainless steel), and biotolerant (polymethylmethacrylate). By using a cDNA microarray containing 687 human IMAGE sequences, we identified in primary cultures of osteoblastic cells differentiated from the human bone marrow and exposed to these biomaterials, genes whose expression was significantly upregulated or downregulated. Among the differentially expressed genes we have found those involved with cell cycle regulation, cell differentiation and proliferation, apoptosis, cell adhesion, bone mineralization and skeletal development. These results can be relevant to a better understanding of the molecular mechanism underlying the behavior of osteoblasts in bone regenerative procedures. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009
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