Three-dimensional imaging of proton gradients at microelectrode surfaces using confocal laser scanning microscopy
2002; Elsevier BV; Volume: 4; Issue: 11 Linguagem: Inglês
10.1016/s1388-2481(02)00482-4
ISSN1873-1902
AutoresSusan Cannan, I. Douglas Macklam, Patrick R. Unwin,
Tópico(s)Force Microscopy Techniques and Applications
ResumoThe first use of fluorescence confocal laser scanning microscopy (CLSM) to image three-dimensional pH gradients at electrode surfaces is described, using the reduction of benzoquinone (BQ) to hydroquinone in aqueous solution as an example. The associated local pH changes accompanying the process have been imaged using a trace amount of fluorescein, which has a pH-dependent fluorescent signal. Images recorded in x–y–z space, allow pH profiles to be obtained as a function of applied electrode potential. Experimentally determined profiles measured when BQ is reduced at a diffusion-limited rate are shown to be in good agreement with predictions from numerical simulation. Future applications of CLSM for pH imaging at electrode surfaces and its use in scanning electrochemical microscopy (SECM) are highlighted briefly.
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