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A colorimetric method for determination of γ-glutamyl-S-ethenyl-cysteine in narbon vetch (Vicia narbonensis L.) seeds

2011; Elsevier BV; Volume: 418; Issue: 2 Linguagem: Inglês

10.1016/j.ab.2011.07.010

1096-0309

R. Sánchez‐Vioque, M.F. Rodríguez-Conde, Javier Vioque, Julio Girón‐Calle, Omar Santana, M. De-Los-Mozos-Pascual, M.E. Izquierdo-Melero, Manuel Alaiz,

Potato Plant Research

A new colorimetric method based on the bleaching of the iodoplatinate ion has been developed for fast and easy determination of γ-glutamyl-S-ethenyl-cysteine (GEC) in narbon vetch (Vicia narbonensis L.) seeds. The calibration curve showed a good correlation (r2 = 0.9959) between absorbance and GEC amounts from 5.5 to 33 μg (10–59.78 μmol/L). The limits of detection and quantification were 1.16 and 3.55 μmol/L, respectively, and no significant interferences from other sulfur-containing compounds were observed. The method showed excellent repeatability (relative standard deviation [RSD] = 0.28%), reproducibility (RSD = 4.4%), and accuracy (94%). Determination of GEC in 20 narbon vetch accessions yielded values that were in agreement with those reported previously using capillary electrophoresis and high-performance liquid chromatography methods. The method could be especially valuable for determination of GEC during the process of production of new low-GEC narbon vetch varieties.