Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

The Significance of p53 Immunoexpression with Different Clones (DO-7 and PAb-240) in Oral Squamous Cell Carcinoma

2013; Karger Publishers; Volume: 75; Issue: 2 Linguagem: Inglês

10.1159/000349978

1423-0275

José de Assis Silva Júnior, Danielle Resende Camisasca, Vagner Gonçalves Bernardo, Georgina Severo Ribeiro, Fernando Luiz Dias, Paulo Antônio Silvestre de Faria, Lı́dia Maria da Fonte de Amorim, Simone de Queiroz Chaves Lourenço,

Salivary Gland Tumors Diagnosis and Treatment

<b><i>Background/Aim:</i></b> The <i>TP53</i> gene is a tumor suppressor gene. Its product is a nuclear protein that regulates cell cycle arrest, apoptosis and DNA repair. Anti-p53 clones DO-7 and PAb-240 recognize the amino acid sequences 21-25 and 213-217, respectively. This study aimed to evaluate the expression of these clones and their relationship with clinicopathological features and survival analysis in oral squamous cell carcinomas (OSCC). <b><i>Methods:</i></b> Information on 53 primary OSCC was collected at the Brazilian National Cancer Institute. An immunohistochemical method was applied to evaluate p53 expression (DO-7 and PAb-240). Their expression was analyzed quantitatively and correlated with clinicopathological features. Kaplan-Meier survival curves and log rank test were used. <b><i>Results:</i></b> Immunopositivity for DO-7 was present in 64% of the cases, while 58% were positive for PAb-240. There was no correlation between immunoexpression of both antibodies and clinicopathological features or survival analysis. DO-7 expression was significantly higher (p = 0.001) than that of PAb-240. <b><i>Conclusions:</i></b> There were quantitative differences between the expression of the antibodies studied, which may reflect a different specificity of each one. To confirm immunohistochemical results and estimate the true prognostic role of <i>TP53</i> in OSCC, it is important to perform mutation analysis.