Involvement of multiple proteases during Fas‐mediated apoptosis in T lymphocytes
1995; Wiley; Volume: 364; Issue: 2 Linguagem: Inglês
10.1016/0014-5793(95)00370-o
ISSN1873-3468
AutoresS. C. Chow, Marianne Weis, G. E. N. Kass, T. H. Holmstrom, John Eriksson, S Orrenius,
Tópico(s)Immunotherapy and Immune Responses
ResumoThe mechanism of Fas antigen‐mediated apoptosis is at present unclear. We show here that the100,000 × g supernatant from cell lysates prepared from anti‐Fas‐stimulated JURKAT T cells, induces chromatin fragmentation in isolated nuclei with concomitant morphological changes typically seen in apoptosis. The formation of this apoptotic nuclei promoting activity (ANPA) in JURKAT T cells after Fas antigen ligation was blocked by the serine protease inhibitors, TPCK and DCI, and by the interleukin 1‐β‐converting enzyme inhibitor, VAD‐FMK. In addition, chromatin degradation and morphological changes mediated by the ANPA in isolated nuclei were inhibited by TPCK, but not by DCI or VAD‐FMK. These results suggest that Fas‐mediated apoptosis in T cells involves the activation of a cascade of proteases.
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