Modulation of hypothalamic norepinephrine release by atrial natriuretic peptide: involvement of cyclic GMP
1992; Elsevier BV; Volume: 213; Issue: 3 Linguagem: Inglês
10.1016/0014-2999(92)90619-f
ISSN1879-0712
AutoresJaisri Giridhar, Robert W. Peoples, Gary E. Isom,
Tópico(s)Neuroendocrine regulation and behavior
ResumoThe ability of atrial natriuretic peptide (ANP) to modulate K+-stimulated release of [3H]norepinephrine ([3H]NE) from rat hypothalamic slices was investigated. ANP-(1–28) significantly decreased K+-stimulated [3H]NE release in a concentration-dependent manner (maximal inhibition = 22% of control with 100 nM, ED50 = 70 pM). Pretreatment with pertussis toxin did not alter the response to ANP. 8Br-cGMP (10 μM), a cGMP analog, significantly decreased [3H]NE release and when combined with 10 nM ANP-(1–28), an additive effect was observed. Additionally, 3-isobutyl 1-methylxanthine (IBMX) (200 μM), a phosphodiesterase inhibitor, combined with ANP-(1–28) 10 nM, significantly decreased [3H]NE release. These results indicate that ANP-(1–28) modulated release of [3H]NE from rat hypothalamic slices and the effect is most likely mediated by elevation of intraneuronal cGMP.
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