Produção Nacional
Vitellogenins and other haemolymph proteins involved in the oogenesis of Rhynchosciara americana
1982; Elsevier BV; Volume: 12; Issue: 2 Linguagem: Inglês
10.1016/0020-1790(82)90006-3
ISSN1879-2928
AutoresA.G. de Bianchi, Carlos Eduardo Winter, Walter R. Terra,
Tópico(s)Reproductive biology and impacts on aquatic species
ResumoThe electrophoretical analysis of haemolymph proteins from Rhynchosciara americana, in the presence of sodium dodecyl sulphate revealed the occurrence of two vitellogenic polypeptides, V1 and V2, with molecular weights of 240,000 and 220,000 respectively. These polypeptides are synthesized by the adult female fat bodies and are found in the yolk granules. The vitellin molecule is formed at least by the polypeptides V1, V2 and two others, V4 and V5 with molecular weights of 67,000 and 55,000 respectively. All these polypeptides can be stained by PAS and by methyl green, suggesting that they are phosphoglycopeptides. Haemolymph proteins, other than vitellogenin, participate in egg formation, the most prominent being a monomeric larval protein of molecular weight 47,000. These larval proteins are found in the soluble fraction of the eggs and make a massive contribution to the total egg protein.
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