Cloning and characterization of Neisseria meningitidis genes encoding the transferrin-binding proteins Tbp1 and Tbp2
1993; Elsevier BV; Volume: 130; Issue: 1 Linguagem: Inglês
10.1016/0378-1119(93)90348-7
ISSN1879-0038
AutoresM Legrain, V. Mazarin, Scan W. Irwin, Bernadette Bouchon, Marie‐José Quentin‐Millet, Eric J. Jacobs, A.B. Schryvers,
Tópico(s)Bacterial Infections and Vaccines
ResumoGenes tbp1 and tbp2, encoding the transferrin-binding proteins Tbp1 and Tbp2, have been isolated from two strains of Neisseria meningitidis. The tbp2 and tbp1 open reading frames are tandemly arranged in the genome with an 87-bp intergenic region, and the DNA region upstream from the tbp2-coding sequence contains domains homologous to Escherichia coli promoter consensus motives. Nucleotide sequence analysis suggests the existence of a Tbp1 precursor carrying an N-terminal signal peptide with a peptidase I cleavage site and of a Tbp2 precursor with N-terminal homology to lipoproteins, including a peptidase II cleavage site. Comparison of the Tbp1 deduced amino acid (aa) sequences from both strains showed about 76% aa homology, while those of Tbp2 revealed only about 47% aa homology. These comparisons should be extended to other Neisseria strains in order to evaluate further this genetic divergence further.
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