Sensitive response of cultured vascular smooth-muscle cells to cadmium cytotoxicity: comparison with cultured vascular endothelial cells and kidney epithelial LLC-PK1 cells
1996; Elsevier BV; Volume: 89; Issue: 2 Linguagem: Inglês
10.1016/s0378-4274(96)03797-6
ISSN1879-3169
AutoresToshiyuki Kaji, Megumi Suzuki, Chika Yamamoto, Yumiko Imaki, Sawako Miyajima, Yasuyuki Fujiwara, Michiko Sakamoto, Hiroshi Kozuka,
Tópico(s)Mercury impact and mitigation studies
ResumoResponse to cadmium cytotoxicity of cultured bovine aortic smooth-muscle cells was compared with that of cultured bovine aortic endothelial cells and porcine kidney epithelial LLC-PK1 cells. The cell damage was evaluated by morphology and the lactate dehydrogenase leakage assay. It was found that vascular smooth-muscle cells are markedly sensitive to cadmium cytotoxicity. The accumulation of intracellular cadmium was much higher but that of metallothionein was much less in vascular smooth-muscle cells than in LLC-PK1 cells; vascular endothelial cells were in between vascular smooth-muscle cells and LLC-PK1 cells. The content of reduced glutathione was slightly increased by cadmium in all three cell types. The present data suggest that a much lower inducibility of metallothionein with a high accumulation of intracellular cadmium in vascular smooth-muscle cells resulted in a marked sensitivity of the cells to cadmium cytotoxicity. Vascular smooth-muscle cells may be one of the critical target of cadmium toxicity.
Referência(s)